plasmids overexpressing ppar-γ cdna  (Addgene inc)

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: PPARγ was negatively correlated with ERRα in EC tissue. ( A ) The relative mRNA expression of PPARγ and ( B ) the relative mRNA expression of ERRα in EC patients with different FIGO stages. ( C ) Immunohistochemical expression of PPARγ and ERRα in the normal endometrium and EC (×200 & ×400). ( D ) Immunohistochemical expression of PPARγ and ERRα in EC type I and EC type II (×200 & ×400). ( E ) Immunohistochemical expression of PPARγ in the normal endometrium and EC. ( F ) Immunohistochemical expression of ERRα in the normal endometrium and EC. CON: normal endometrium. EC, endometrial cancer. *, P<0.05; #, P>0.05.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Expressing, Immunohistochemical staining

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: Correlation between PPARγ, ERRα expression immunoreactivity in EC.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: Summary of functional annotation and pathway enrichment for PPARγ and ERRα. ( A ) Boxplot showing the relative expression of PPARγ and ERRα in normal and EC samples from the UALCAN database. ( B ) Boxplot showing the relative expression of PPARγ and ERRα in EC based on histological subtypes from the UALCAN database. ( C ) Protein mapping for PPARγ and ERRα based on string data. ( D ) Venn diagram showing the common target genes of PPARγ and ERRα. ( E – G ) GO enrichment of the common target genes of PPARγ and ERRα. ( H ) KEGG enrichment of the target genes regulated by PPARγ and ERRα. ( I ) The ModFit of PPARγ based on the JASPAR database. ( J ) The ModFit of ERRα based on the JASPAR database. ( K ) KEGG pathway maps for apoptosis; The red dots represent target genes of PPARγ and ERRα. ( L ) Possible mechanism of interaction between PPARγ and ERRα. EC, endometrial cancer. *, P<0.05; #, P>0.05.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Functional Assay, Expressing

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: ERRα and PPAR γ negatively regulate each other in EC cells. ( A ) The relative mRNA expression of PPARγ and ( B ) the relative mRNA expression of ERRα in RL952, ECC-1, HEC-1A, and HEC-1B cells. ( C ) Protein expression of PPAR γ and ERRα in RL952, ECC-1, HEC-1A, and HEC-1B cells. ( D ) Upregulated PPARγ and ( E ) suppressed PPARγ by GW9662: the relative mRNA expression of PPARγ and ERRα in EC cells. ( F ) Upregulated PPARγ and ( G ) suppressed PPARγ by GW9662: the protein expression of PPARγ and ERRα in EC cells. ( H ) Upregulated ERRα and (I) suppressed ERRα by XCT790: the relative mRNA expression of ERRα and PPARγ in EC cells. ( J ) Upregulated ERRα and ( K ) suppressed ERRα by XCT790: the protein expression of PPAR γ and ERRα in EC cells. OV-PPARγ, overexpression of PPARγ. OV-ERRα, overexpression of ERRα. EC, endometrial cancer. *, P<0.05; #, P>0.05.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Expressing, Over Expression

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: PPARγ and ERRα compete to control cell proliferation and promote apoptosis in EC cells. ( A ) The effect of OV-PPARγ, OV-ERRα, GW9662 or XCT790 on proliferation. The OD values of HCE-1A and HEC-1B cells were detected at 0, 24, 48, 72, and 96 h after transfection with lentivirus or treatment with ERRα and PPARγ antagonists. ( B , C ) The effect of GW9662 or XCT790 on the cell cycle. HEC-1A and HEC-1B cells were treated with DMSO, GW9662 (5 μM) or XCT790 (10 μM) for 72 h. ( D , E ) The effect of OV-PPARγ or GW9662 on apoptosis.( F , G ) The effect of OV-ERRα or XCT790 on apoptosis. OV-PPARγ: overexpression of PPARγ; OV-ERRα: overexpression of ERRα. EC, endometrial cancer. *, P<0.05; #, P>0.05.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Control, Transfection, Over Expression

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: Diagnostic and prognostic value of the PPARγ/ERRα ratio for EC. ( A ) ROC curves of the mRNA expression of PPARγ, the mRNA expression of ERRα, the PPARγ/ERRα ratio, CA125, CA199, CA153, CEA, and AFP. ( B ) DFS and OS in EC patients with different expression patterns of PPARγ/ERRα. Patients with PPARγ(+)/ERRα(-) had longer DFS and OS. The mRNA expression of PPARγ and ERRα was quantified using the 2 -ΔCT method. PPARγ/ERRα was quantified as the ratio of the mRNA expression of PPARγ to the mRNA expression of ERRα. ROC, receiver operating characteristic; DFS, disease-free survival. OS, overall survival.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: Diagnostic Assay, Expressing

Journal: Aging (Albany NY)

Article Title: ERRα inhibitor acts as a potential agonist of PPARγ to induce cell apoptosis and inhibit cell proliferation in endometrial cancer

doi: 10.18632/aging.104049

Figure Lengend Snippet: Logistic regression model: risk factors for endometrial cancer.

Article Snippet: Full-length cDNA plasmids for PPARγ (PPARG, NM_015869) and ERRα (ESRRA, NM_004451) were purchased from Genechem (Shanghai, People's Republic of China) and cloned into a lentivirus-based vector carrying the green fluorescent protein (GFP) gene (GV358, Genechem, Shanghai, China) with the following component sequence: Ubi-MCS-3FLAG-SV40-EGFP-IRES-puromycin.

Techniques: